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Reduced NNMT in adipose tissue was associated with increased expression of polyamine flux genes and increased adipocyte energy expenditure connecting NNMT inhibition to "futile cycling" mechanisms that consume energy without productive metabolic output

M-mode measurements of the LV cavity size in systole and diastole were made, and global LV function was assessed by calculating ejection fraction (EF) from end-diastolic and end-systolic volumes of the left ventricle according to the following equations: diastolic volume (DV) (mL) = 0.001047 left ventricle internal dimension in diastole (LVIDd (mm)) (Balenovic et al., 2009)

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