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Seven days after culturing, an Electric Cell-Substrate Impedance Sensing (ECIS) device is utilized to observe physiological changes and migration of cancer cells

Duration mattered: shorter studies that found no effect may simply have not run long enough

doi: 10.3390/life13091845

when cellular energy is sufficient, mTORC1 is activated to drive cell proliferation (90), whereas low ATP levels activate AMPK, which phosphorylates TSC2, stabilizing the TSC complex and inhibiting mTORC1 (91)