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For testing the difference in mean and median lifespan between groups, we used linear mixed model with linear quantile mixed-effects model, which included cage assignment as a random effect and batch as a covariate, respectively, using package nlme and lgmm in statistical software R

the dosage forms are all subcutaneous/intramuscular injections, and to avoid serious gastrointestinal adverse events, it is necessary to start with a low dosage and gradually increase the dosage during use

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