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Fluorescence staining Oocytes were incubated in M2 medium containing 10 M H2DCFDA (MCE, USA), 5 M MitoSOX Red (MCE, USA), and 10 g/mL Hoechst 33342 (Beyotime, China) at 37C in a 5% CO 2 atmosphere for 15 min

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While collectively these findings suggest that ghrelin and GLP-1 signaling have opposing effects on FAA and conditioned eating, more preclinical rodent work is needed to understand the functional role of premeal GLP-1 release, as well as the larger role of GLP-1 in meal anticipatory behavior