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Samples were boiled for 5 min at 95 C with loading buffer (10% 2-mercaptoethanol, 4%SDS, 20%glycerol, 0.125M Tris HCl, and 0.004% bromophenol blue PH6.8)

Environmental xenobiotics and the antihormones cyproterone acetate and spironolactone use the nuclear hormone pregnenolone x receptor to activate the CYP3A23 hormone response element