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Some authors have theorised that the mechanisms underlying these observed associations are either as a result of a transfer of genes from smoking parents to their children, thereby increasing their liability to smoke and subsequently use other drugs 23 and/or the imitation of particular personality characteristics of parents 24

Indeed, while primary cultures may better mimic a physiological behavior, they are associated with technical challenges (lack of tissue availability, specific handling required and donor-specific variations) [19, 40]

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This could be attributed to the fact that the average user consumes can per day or 8-12 nicotine pouches