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Cells were washed with PBS and incubated with nicotine (31.25 ng/ml400 ng/ml) or CSS as above for a further 24 h or 48 h (A549 cells), then measured as nitrite and nitrate (NOx) accumulation in media as described previously [2325]

Youre trying to thread the needle. Policies specifically targeted at youth use might be more effective, such as only allowing the sale of flavored nicotine liquid at stores that only serve customers 21 and up, Wagner said

The overall impact on sales is expected to be small however, local retailers said, because New York and New Jersey have had higher tax rates on cigarettes for years and few can afford to travel across state lines just to buy cigarettes

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